Confocal micrograph of mouse small intestine with the Red2Onco technology. With Red2Onco, the oncogenic mutant clones (Red clones), and normal or wild-type clones (Yellow or Cyan clones) can be labeled in the same tissue. The mutant clones expand considerably faster than wild-type clones, suggesting field expansion of the mutant clones. Images acquired after 6 months of tissue lineage tracing. Credit: Yum/IMBA
Red2Onco, an innovative genetic mouse model, allows to detect the very initial steps that lead to cancer development. Red2Oncos multi-color labeling system allows to trace intestinal tumor development after the first oncogenic hit at the single cell level. The research carried out at IMBA Institute of Molecular Biotechnology of the Austrian Academy of Sciences and the University of Cambridge is now published in the journal Nature.
Studies on cancer are limited by the threshold at which cellular transformations become clinically detectable. However, the very initial phase on the way to malignancy is histologically invisible, as the process originates from one single cell. In this early phase, a so-called seeding cell acquires an initial pro-cancerous mutation, also known as the first oncogenic hit, while being completely surrounded by normal tissue. To overcome the detection barrier, a team of researchers around IMBA group leader Bon-Kyoung Koo and University of Cambridge group leader Professor Benjamin D. Simons developed a laboratory system to dissect the pre-cancerous steps that remained under the radar up until present.
Another confocal micrograph of mouse small intestine with the Red2Onco technology. Credit: Yum/IMBA
With the advance of technologies like deep targeted DNA sequencing, researchers noticed that cancer-associated mutations are already present in normal tissues, which is very scary! states Bon-Kyoung Koo, referring to the turning point that set him, like a forensic detective, on the traces of the first oncogenic hit.
As most human cancer types originate in the epithelium, Koo, a geneticist, decided to build a genetic model to examine the effect of the first oncogenic transformations in the mouse gut. This genetic model, which the team termed Red2Onco, is a multi-color labeling technology that allows to track the initiation of tumorigenesis from a single mutant cell. With Red2Onco, the team found that mutant cells create a hostile environment to their neighboring non-mutant cells and massively deregulate the normal stem cell niche in the mouse intestinal tissue.
This is by far the most exciting approach that we have tried! states Bon-Kyoung Koo.
Another confocal micrograph of mouse small intestine with the Red2Onco technology. Credit: Yum/IMBA
Using Red2Onco, the team was able to study the mechanisms initiated by two separate first oncogenic hits. These are mutations to known proto-oncogenes, KRAS and PI3K, respectively, being known as pro-cancerous factors driving malignancy in declared tumors. To their surprise, the researchers found that even in the event of such a first oncogenic hit, the mutated pre-cancerous cell or seeding cell imparts a negative influence on its neighbors. The surrounding normal tissue loses its stem cells, which in turn favours the territorial expansion of oncogenic mutant stem cells and their progenies. Through this process of field transformation, the colonization of intestinal tissue by mutant cells increases the chance for further oncogenic hits, which may lead in turn to cancer, explains Benjamin Simons of the Gurdon Institute, University of Cambridge.
The first author on the study, Min Kyu Yum of the Gurdon Institute, expands: oncogenic mutant cells influence the fate behavior of their wild-type neighbors both directly, through secretion of signaling factors, and indirectly through induced changes in the shared tissue environment, before co-first author Seungmin Han sums up: Using comparative single-cell analyses and organoid culture methods, we were able to dissect the molecular mechanisms that mediate cellular crosstalk.
The relevance of the work is major, to say the least. In this sense, the authors have shown that inhibiting BMP (Bone Morphogenic Protein) signaling coming from the oncogenic mutant relieves the negative influence on the normal stem cells. As well as enabling detection of early events of tumorigenesis, our discovery paves the way for intervention strategies that target cellular crosstalk mechanisms, concludes Min Kyu Yum.
Reference: Tracing oncogene-driven remodelling of the intestinal stem cell niche by Min Kyu Yum, Seungmin Han, Juergen Fink, Szu-Hsien Sam Wu, Catherine Dabrowska, Teodora Trendafilova, Roxana Mustata, Lemonia Chatzeli, Roberta Azzarelli, Irina Pshenichnaya, Eunmin Lee, Frances England, Jong Kyoung Kim, Daniel E. Stange, Anna Philpott, Joo-Hyeon Lee, Bon-Kyoung Koo and Benjamin D. Simons, 2 June 2021, Nature.DOI: 10.1038/s41586-021-03605-0
Originally posted here:
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